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Conventional procedure to study and explore the large size gene or gene clusters is based on library construction and screening,which is laborious.A straightforward strategy has been developed to clone these gene or gene clusters from the linear genomic DNA pool.This new DNA cloning technology,named Direct Cloning1,allows cloning of up to 60kb DNA fragment from genomic DNA in one step and in two days.DNA direct cloning technology is mediated by full-length RecET not lambda phage proteins Red.It is a new homologous recombination method different than recombineering2 which mediated by truncated RecET and lambda Red.