【摘 要】
：
orexin (orexinA和B,又称hypocretin 1和2)是产生和维持哺乳动物觉醒的一种重要的促觉醒肽.Orexin神经元胞体主要位于穹隆周的外侧下丘脑区,其纤维末梢和受体广泛分布于中枢神经系统(CNS).研究显示,orexin系统统一调控脑内众多而分散的觉醒调节系统.因此,orexin系统被认为是觉醒状态发生和维持的关键性结构,对orexin神经元活动调节的研究就显得尤为必要.腺苷是
【机 构】
：
第三军医大学基础部生理学教研室,重庆400038 中国科学院神经科学研究所,上海200031
【出 处】
：
中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the
论文部分内容阅读
orexin (orexinA和B,又称hypocretin 1和2)是产生和维持哺乳动物觉醒的一种重要的促觉醒肽.Orexin神经元胞体主要位于穹隆周的外侧下丘脑区,其纤维末梢和受体广泛分布于中枢神经系统(CNS).研究显示,orexin系统统一调控脑内众多而分散的觉醒调节系统.因此,orexin系统被认为是觉醒状态发生和维持的关键性结构,对orexin神经元活动调节的研究就显得尤为必要.腺苷是目前公认的、研究最多、最重要的睡眠稳态调控因子,能够促进由觉醒状态向睡眠状态的过渡.最新研究显示,腺苷可以抑制orexin神经元的活动,但是这种调控效应的机制以及参与此效应的腺苷来源尚不清楚.本研究中,我们利用orexin-EGFP转基因小鼠,采用脑片膜片钳技术,观察腺苷对orexin神经元活动的影响.研究结果显示,腺苷(100 μmol/L)对orexin神经元的诱发兴奋性突触后电流(evoked EPSC)有抑制效应,这与先前的文献报道相一致.同时,我们发现腺苷对主要由GABA介导的orexin神经元抑制性突触后电流(evoked IPSC)也有显著的抑制效应.腺苷对orexin神经元evoked lPSC的抑制效应呈现明显的剂量依赖性：100 μmol/L,50μmol/L,25 μmol/L,10 μmol/L腺苷对orexin神经元evoked IPSC的抑制效应分别为60.93％,70.49％,79.21％,和84.70％.
其他文献
AIM During the inflammatory processes in CNS,activated microglia can be induced by many factors.Activation of microglia induces the release of some neurotoxic substances such as nitric oxide (NO),whic
Background Various mammalian species,including human being,have neural stem cells (NSCs) in central nervous system all theirlife.NSCs are capable of generating various kinds of cell within the central
Endocytosis of tyrosine receptor kinases (Trks) is involved in neurotrophin-dependent neuronal survival.However,the mechanisms governing the endocytosis of TrkB and its specific contribution to TrkB d
Long-term Plasticity is an important hypothesis for learning and memory.As disclosed from many experimental data,an activation of NMDA receptors is required for long-term potentiation (LTP) and long-t
Alzheimers disease (AD) is the most prevalent neurodegenerative disease in aged people leading to progres-sive loss of memory and cognitive deficits.One of the major pathological features in AD brain
It is well known that obstructive sleep apnea and associated intermittent hypoxemia cause deficit in learning and memory in human.We had shown previously that intermittent hypoxia (IH) impairs long-te
The main pathological characteristics of Alzheimers disease (AD) is the high density of senile plaques (SPs) in the brain which constitute of amyloid β-peptide (Aβ).However,the possible mechanisms by
Neural stem cells/neural precursors (NSCs/NPs) may play important roles in transplantation therapies for neurodegenerative diseases,but the mechanism of NSCs/NPs differentiation is poorly understood.H
Alzheimers disease (AD) is the most common cause of dementia in the elderly,characterized mostly by cognitive deficits and progressive loss of memory.Amyloid β (Aβ) protein is widely thought to play a
Objective To study the effects of fimbria fornix transection and intraventricular injection of nerve growth factor (NGF) on the proliferation and differentiation of endogenous neural stem cells (NSCs)