Molecular characterization of lipoxygenase genes on the chromosome of 4BS in Chinese bread wheat (Tr

• 作 者: Zhang Fuyan Wu Peipei Chen Feng Cui Dangqun
• 机 构: Agronomy College/Collaborative Innovation Center of Henan Grain Crops/National Key Laboratory of Whe
• 出 处: 第六届全国小麦基因组学及分子育种大会
• 发表日期: 2015年5期
• 关键词: Bread wheat Lipoxygenase genes Lox activity Allelic variation

　　Lipoxygenase(Lox),a critical enzyme in the carotenoid biosynthetic pathway,has a profound influence on the color and processing quality of wheat-based products.In this study,two novel Lox genes,designated TaLox-B2 and TaLox-B3,were cloned on chromosome4BS of Chinese bread wheat.The TaLox-B2 with 4267 bp and TaLox-B3 gene with 4246 bp are composed of seven exons and six introns.The deduced amino acid sequence showed that both TaLox-B2 and TaLox-B3genes encoded an 861-aa protein and possessed a lipoxygenase superfamily domain at the 170-838 interval.Subsequenctly,two different TaLox-B2 alleles,designated TaLox-B2a and TaLox-B2b,were discovered in diverse Chinese cultivars.A co-dominant marker Lox-B23 was developed based on sequences of TaLox-B2a,TaLox-B2b and TaLox-B3 genes in order to more precisely distinguish these three alleles in Chinese bread cultivars.Amongst five allelic combinations of Lox genes at Lox-B1,Lox-B2 and Lox-B3 loci,wheat cultivars with the TaLox-B1a/TaLox-B2a/TaLox-B3a combination had the highest Loxactivity,whereas the wheat cultivars with TaLox-B1a/TaLox-B2b/TaLox-B3b combination had significantly lowest Loxactivity.A RIL population was used to evaluate the influence of TaLox-B3a gene on Lox activity.Results showed that TaLox-B3a gene could significantly increase the Lox activity in bread wheat.Physical mapping indicated that both TaLox-B2 and TaLox-B3 genes were located on chromosome 4BS in bread wheat.This study provides useful information to further understand the molecular and genetic bases of Lox activity in bread wheat.