【摘 要】
：
小麦返白系受持续低温诱导后表现自心叶至整株白化,气温回升后则迅速复绿。在田间,白化阶段的返白系群体出现白化株和绿株的表型差异,且同一植株的不同分蘖会表现白化和绿苗的差异,据此推测白化现象与表观遗传的调控有 关。对基因芯片及数字表达谱结果分析、并经Real time PCR结果验证表明,持续低温条件下,返白系的组蛋白去乙酰化酶(histone deacetylase,HDAC)基因HDAC3、HDA
【机 构】
：
西北农林科技大学生命科学学院,陕西杨凌712100 西北农林科技大学生命科学学院,陕西杨凌7121
【出 处】
：
第六届全国小麦基因组学及分子育种大会
论文部分内容阅读
小麦返白系受持续低温诱导后表现自心叶至整株白化,气温回升后则迅速复绿。在田间,白化阶段的返白系群体出现白化株和绿株的表型差异,且同一植株的不同分蘖会表现白化和绿苗的差异,据此推测白化现象与表观遗传的调控有 关。对基因芯片及数字表达谱结果分析、并经Real time PCR结果验证表明,持续低温条件下,返白系的组蛋白去乙酰化酶(histone deacetylase,HDAC)基因HDAC3、HDA C2、HDAC4,类拟南芥AtSin3基因,DNA甲基化转移酶基因,组蛋白基因等的表达与母本矮变1号相比,存在显著差异。其中,HDAC3与Sin3表达模式相近,在低温白化与升温复绿过程中,均是返白系中的表达量高于矮变1号,并且升温复绿后的返白系植株与白化苗相比表达下调。该结果揭示,返白系中低温诱导的组蛋白去乙酰化修饰可能高于矮变1号,与叶绿体发育受阻和叶色白化相关。在拟南芥中,SIN3是转录共抑制子,参与ABA介导的表观遗传,HDAC/SIN3复合体与蛋白激酶PKS3结合激活转录因子AtERF7,与启动子GCCbox区结合,最终引起组蛋白的去乙酰化,抑制基因的转录、降低植物体对ABA的反应和对胁迫的耐受性。小麦HDAC3与拟南芥HDACs/SIN3复合体中的AtHDA6和AtHDA 19氨基酸序列有较高的同源性(75％),我们推测,在返白系中也存在HDAC3/SIN3复合体,通过组蛋白去乙酰化调控植株对ABA及低温的响应。
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