A3DPrintedPLGA/HAScaffoldGraftedwithQuaternisedChitosan forEnhancementofAnti-BiofilmFor

• 作 者: YingYang Sheng-BingYang Hai-YongAo Ting-TingTang
• 机 构: ShanghaiKeyLaboratoryofOrthopaedicImplant,DepartmentofOrthopaedicSurgery,ShanghaiNinthPe
• 出 处: 中华医学会第十七届骨科学术会议暨第十届COA国际学术大会
• 发表日期: 2015年4期
• 关键词: Bioactivecompositeporousscaffold quaternisedchitosan bacteriaadhension biof

　　Infectiousbonedefectremainsaseriouscomplicatedchallengeincurrentorthopaedicsurgery.Fortreatmentoflargebone defectedcombinedwithintractableinfection,thelocaluseofantibiotic-loadedpolymethylmethacrylate(PMMA)bonecements,includinggentamicinandhydroxypropyltrimethylammoniumchloridechitosan(HACC),areinsufficientforbonerepair.Astherapeuticagentsaredesirableforenhancementofantibacterialandosteogenicactivity,thisstudywasdesignedtodevelop sucha3Dprintedbioactiveporousscaffold(PLGA/10%HA/HACC)madeofpolylactide-co-glycolide(PLGA)andhydroxyapatite(HA)(weightratio,PLGA:HA=9:1)asfundamentalcarrier,graftingwithHACCwitha22%degreeofsubstitution.PLGA,PLGA/10%HAandPLGA/HACCscaffoldswerealsoinvestigatedandcompared.Threestandardstrains,Staphylococcusaureus(ATCC25923), Staphylococcusepidermidis(ATCC35984)andmethicillin-resistantStaphylococcusaureus(ATCC43300),andoneclinicalisolates, methicillin-resistantS.epidermidis(MRSE287),wereselectedtoevaluatedthebacterialadhesionandbiofilmformationat4,24and48husingthespreadplatemethod,tissuecultureplate(TCP)methodandconfocallaserscanningmicroscopy(CLSM)in vitro.WefoundthatPLGA/10%HA/HACCandPLGA/HACCcompositescaffoldscouldsignificantlyinhibitbacterialadhesionand biofilmformationcomparedtoPLGAandPLGA/10%HAscaffolds,andscaffoldscontainHAshowedrelativehigherbacterialadhesiontosomeextent.Meanwhile,humanmarrowderivedmesenchymalstemcellswereusedtoassesstheosteogenicandrecruitmentactivityofcombinedapplicationofHAandHACCinPLGAscaffold.OurefficacystudyshowedthatPLGA/10%HAand PLGA/10%HA/HACCscaffoldsdemonstratedsignificantlypromotedcellattachment,proliferation,spreading,osteogenicdifferentiationandmigrationwhencomapredtothePLGAandPLGA/HACCscaffolds.Furthermore,aninvivodorsum subcutaneousembeddingmodelinSDratsusingabioluminescentS.aureus(ATCC12600,Xen29)wasalsoestablishedforrealtime monitoringofbacterialinfectionbytheIVISImagingSystem.TheinvivoobservationshowedanevidentdecreaseinbothluminescenceandbacterialcountsandunconspicuoussubcutaneousabscessinthegroupsofPLGA/10%HA/HACCandPLGA/HACCcomparedtotheothertwogroups.Ingeneral,thesefindingsmaydevelopagoodfoundationforpotentialclinicalvalidationofthisinovativebioactiveporousscaffoldforcombatinginfectiousbonedefect.