【摘 要】
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[Purpose] Nasopharyngeal carcinoma (NPC) is the most common malignant tumor in Southeast Asia and radio-resistance remains a serious obstacle to successful treatment.Curcumin has been used to treat NP
【机 构】
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Molecular Pharmacology Research Center,School of Pharmacy,Wenzhou Medical University,Wenzhou 325035,
【出 处】
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2013抗肿瘤药物研究新进展与肿瘤个性化药物治疗论坛
论文部分内容阅读
[Purpose] Nasopharyngeal carcinoma (NPC) is the most common malignant tumor in Southeast Asia and radio-resistance remains a serious obstacle to successful treatment.Curcumin has been used to treat NPC.[Methods] A new curcumin analogue, (1E, 4E)-1,5-bis (2-bromophenyl)penta-1,4-dien-3-one (B6) was synthesized.We compared the effect of B6 and curcumin in radiosensitive cells CNE2 and radio-resistant cells CNE2R on proliferation, apoptosis, cell cycle, protein expression on endoplasmic reticulum (ER) stress and subcutaneously inoculated NPC tumor models of nude mice.CHOP silencing by specific siRNA was used.[Results] The growth inhibitory, G2/M arrest and inducing apoptosis effect of B6 was more potential than curcumin in vitro and in vivo.CNE2R was more sensitive to B6 treatment than CNE2.B6 induced ER stress response, up-regulation CHOP, XBP1, ATF4 and GRP78 protein expression in two cell lines, while curcumin having no effect.Suppression of CHOP expression with siRNA efficiently reduced the apoptosis induced by B6.[Conclusion] B6 has more potent anti-tumor activity than curcumin and enhances the ability in radio-resistant cells, which is associated with activation of ER stress.
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