【摘 要】
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Objective To investigate the effects of PTEN and KAI1 double overexpression on proliferation, metastasis and radio-sensitivity in ASPC-1 pancreatic cancer cells under hypoxic condition.Methods Recombi
【机 构】
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Department of Gastroenterology, General Hospital of Shenyang Military Area
【出 处】
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The 14th Congress of Gastroenterology China(第十四届全国消化系病学术会议)
论文部分内容阅读
Objective To investigate the effects of PTEN and KAI1 double overexpression on proliferation, metastasis and radio-sensitivity in ASPC-1 pancreatic cancer cells under hypoxic condition.Methods Recombinant vectors that over-express PTEN and KM1 genes respectively were transfected into hypoxic ASPC-1 cells.Westem blot was performed to detect the expression level of them.Then, cell growth curve assay by MTT and colony-forming assay were used to test the proliferation, and transwell assay was performed to evaluate metastatic ability.Annexin V flow cytometry was used to determinate the apoptosis rate of X-ray treated ASPC-1 cells, which may reflect the influence on radio-sensitivity after PTEN and KAI 1 double transfection.
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