多巴胺D2样受体激动对多巴胺能细胞自噬的调控及其机制研究

来源 :第一届自噬生物学与疾病国际研讨会(The 1st International Symposium on Autophag | 被引量 : 0次 | 上传用户：zybp821

【摘要】

：

【作者】

：

王健达曹钰兰刘春风胡丽芳

【机构】

：

Department of Neurology,Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psych

【出处】

：

第一届自噬生物学与疾病国际研讨会(The 1st International Symposium on Autophag

【发表日期】

：

2015年9期

下载到本地 , 更方便阅读

下载此文赞助VIP

声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架

论文部分内容阅读

离体实验中,我们发现多巴胺D2样受体激动剂(普拉克索和喹吡罗)可使PC12、MES23.5、诱导分化的SH-SY5Y细胞内LC3 Ⅱ、Beclin 1蛋白水平增加,P62蛋白水平下降.但普拉克索的对映体(几乎无多巴胺受体激动活性)和未分化的SH-SY5Y(多巴胺D2样受体表达较低)未观察上述现象.特异性多巴胺D2、D3受体抑制剂存在时,普拉克索不能促使多巴胺能细胞中上述自噬相关蛋白水平增加.此外,普拉克索可使PC12细胞内钙离子及c-Fos蛋白含量增加.清除细胞内钙离子或干扰e-Fos后,普拉克索则无法提高PC12细胞的自噬水平.染色质免疫共沉淀证实c-Fos可与Beclin 1上游某个潜在的AP-1 (TGCCTCA)位点结合.荧光素酶双标实验发现普拉克索可使Beclin 1启动子活性增加,去除这个潜在的AP-1位点后则无法增加.

其他文献

Analysis, prevention and treatment strategies for intraoperative iatrogenic injury of anterior prepe

Objectives To investigate the etiology, prevention and treatment strategies for intraoperative iatrogenic injury of anterior preperitoneal groin hernia repair.Methods A retrospective review of 12 pati

会议

Groin herniaPreperitoneal herniorrhaphyIatrogenic injuryComplication

A comparative experimental study of the influence of different meshes on the structure of vas defere

Objective Explore the influence of heavyweight polypropylene mesh, lightweight polypropylene mesh and polyester mesh on the structure of vas deferens at the different time in rat models.Methods There

会议

HerniaRatMeshVas deferensReproductive functions

Negative pressure wound therapy for mesh-site infection after incisional hernia repair: a case repor

Purpose We reviewed a case of negative pressure wound therapy (NPWT) for mesh-site infection after incisional hernia repair and relevant literature.Methods This was a 76 years old female patient.She h

会议

Negative pressure wound therapyInfected mesh graftIncisional hernia repair

Biologic Mesh versus Synthetic Mesh in Open Inguinal Hernia Repair: System Review and Meta-Analysis

Background Biological meshes are mostly used for abdominal wall reinforcement in infected fields, but no consensus has been reached on its use for inguinal hernia repairing.The purpose of this study w

会议

Biologic meshSynthetic meshOpen inguinal hernia repair

信号衔接蛋白p66Shc的缺失抑制营养缺乏诱导的细胞自噬

失巢凋亡指失去胞外贴附基质而引发的细胞死亡,实体肿瘤细胞耐受失巢凋亡是产生转移的生物学基础之一.细胞自噬是一种高度保守的依赖溶酶体降解受损大分子或细胞器的自我消化过程,从而循环利用降解产物.在实体肿瘤细胞转移过程中,细胞自噬起着不可或缺的作用.p66Shc是信号衔接蛋白ShcA的一个异构体,在正常表皮细胞中高表达,但在转移的非小细胞肺癌细胞中低表达或不表达,临床肺癌组织样品中其表达与患者存活时间负

会议

BMA 155 induces autophagy and following apoptosis associated with NF-κB Pathway in human hepatocarci

Bisindolylmaleimide alkaloid-155 (BMA155) is a synthesized bisindolylmaleimide compound with novel structure.We showed here that BMA155 induced autophagy and following apoptosis associated with NF-κB

会议

A novel ECG analog selectively induces apoptosis of B16-F10 melanoma via activation of autophagy and

Autophagy-induced cancer cell death has become a novel signaling target for development of cancer therapeutic drugs.Green tea polyphenols induced both autophagy and apoptosis in a variety of cancer ce

会议

bFGF regulates autophagy and ubiquitinated protein accumulationinduced by myocardial ischemia/reperf

Autophagy is involved in the development and/or progression of many diseases, including myocardial ischemia/reperfusion (I/R).In this study, we hypothesized a protective role of basic fibroblast growt

会议

Viral protein-repressed microRNA-148a controls tumor growth and metastasis through HPIP-mediated mTO

在病毒引发的相关肿瘤中miRNA的表达异常已有芯片数据报道,然而,在细胞内miRNA通过何种信号通路调控病毒相关肿瘤的发生和发展我们所知甚少.本研究发现miR-148a/HPIP/mTOR信号通路在调控病毒相关肿瘤的生长和转移的作用.我们首先发现HPIP为miR-148a的直接靶基因,miR-148a会降低HPIP的表达,其表达降低导致AKT和ERK通路的抑制,进而又抑制了在自噬中发挥重要作用的m

会议

Melatonin attenuates MPTP-induced neurotoxicity via preventing CDK5-mediated autophagy and α-synucle

Autophagy is involved in the pathogenesis of neurodegenerative diseases including Parkinsons disease (PD).However, little is known about the regulation of autophagy in neurodegenerative process.In thi

会议

多巴胺D2样受体激动对多巴胺能细胞自噬的调控及其机制研究

其他学术论文