【摘 要】
：
A high-performance liquid chromatography coupled with electrospray ionization mass spectrometry method was presented for the simultaneous determination of five synthetic nonsteroidal anti-inflammatory
【机 构】
：
Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical
【出 处】
：
2009上海中药与天然药物国际大会
论文部分内容阅读
A high-performance liquid chromatography coupled with electrospray ionization mass spectrometry method was presented for the simultaneous determination of five synthetic nonsteroidal anti-inflammatory drugs as well as two glucocorticoids,i.e.,piroxican,prednisone acetate,dexamethasone acetate,phenylbutazone,diclofenac,indometacin,and ibuprofen in twelve Chinese proprietary medicines for rheumatism.Glipizide was used as the internal standard.The separation was completed on a C18 column with the mobile phase consisted of methanol and ammonium acetate buffer (20 mM ammonium acetate and 0.1% formic acid) with gradient elution.The eight analytes were separated on baseline for preliminary qualitative assay and quantitative analysis.Full validation of the developed quantitative method was provided (linearity,limit of detection,limit of quantification,precision and accuracy) by HPLC-UV.LC-ESI-MS method was applied to further identify the structures of the analytes.The proposed method was suitable for safety assurance and quality control of suspect Chinese proprietary medicine for rheumatism.
其他文献
The European Pharmacopoeia Commission has initiated a work programme dedicated to herbal drugs used in Traditional Chinese Medicines in order to take account of the importance and the growing interest
Biosilicifieation (formation of biosilica) has become an exciting source of inspiration for the development of novel bionic approaches.Silica is an important material in nanotechnology.Conventional pr
Countercurrent chromatography (CCC) is a liquid-liquid purification technology with both liquid mobile and stationary phases.This feature provides the best conditions for handling herbal crude extract
In recent years,we have developed an analytical tool box in our lab to fit for variety of small molecule sample profiling applications.In this tool box,the hardware tools such as UPLC,oa-TOF MS,ion mo
Src family kinases (SFKs) are constitutively activated in numerous human cancer cell lines and tumor tissues of patients,resulting in cell proliferation,tumorigenesis and metastasis.SFKs phosphorylate
本文首次采用UPLC-PDA同时测定白屈菜中7个主要生物碱成分,包括：原阿片碱、白屈菜碱、黄连碱、血根碱、小檗碱、四氢黄连碱、白屈菜红碱.采用ACQUITY UPLC BEHC 18(2.1×100 mm,1.7μm)色谱柱,流动相由乙腈(A)-醋酸-醋酸铵缓冲液(pH 3.0；10mM) (B)梯度组成,达到基线分离.所有标准曲线均呈良好线性关系(r＞0.9992),该方法具有较好的精密度和回收
槲皮素作为一种重要的黄酮类天然产物在食物中含量能达到10 mg/kg,其具有较强的抗氧化活性,在肿瘤预防方面具有较强的开发前景。对槲皮素体内代谢研究发现,在体内主要有三种代谢方式,分别是甲基化、硫酸酯化和葡萄糖醛酸化。对猪喂养含有丰富槲皮素的食物后研究发现,其血浆没有原型槲皮素存在,血浆中的主要代谢物包括3’-O-甲基槲皮素(isorhamnetin)、4’-O-甲基槲皮素(tamarixetin
Moutan Cortex is a well-known herb in traditional Korean,Chinese,and Japanese anti-diabetic formulae.In the current study,we investigated the metabolic effects of isolated triterpenes (1-7) in HepG2 c