论文部分内容阅读
To study the molecular epidemiological characteristic of hepatitis E virus isolates from year 2000-2003 in Fujian Province.Nested RT-PCR was carried out to amplify the HEV gene of open reading frame2 (ORF-2).The purified PCR products were cloned and then sequenced.Phylogenetic analysis was performed by Neighbor-Joining and Bootstrap.HEV RNAs were amplified in 72 sera from 158 ptients with acute sporadic hepatitis E, all of which were characterized as HEV genotype Ⅳ, and could be dvided into four distinct groups (A,B,C and D).