【摘 要】
：
The achievement of successful pregnancy in humans by intracytoplasmic sperm injection (ICSI) was first reported by Palermo et al.in 1992.Since then, ICSI has been used as an essential technique in ass
【机 构】
：
Fertility Clinic Tokyo Japan
【出 处】
：
BIT`s 1st International Congress of Gynaecology and Obstetri
论文部分内容阅读
The achievement of successful pregnancy in humans by intracytoplasmic sperm injection (ICSI) was first reported by Palermo et al.in 1992.Since then, ICSI has been used as an essential technique in assisted reproductive technologies.However, there has been concern since the inception that natural selection in fertilization might be eliminated because the fertilization process in ICSI, which is performed when fertilization fails in IVF, involves many artificial elements.Spermatozoa used for intracytoplasmic sperm injection (ICSI) are selected based on their motility and morphology.To explore other methods for selecting better spermatozoa, we developed a spermatozoa-sorting method using a physiologically natural selection system involving penetration into cervical mucus (CM).In addition, we analyzed the spermatozoa that penetrated the CM (CMpenetrating spermatozoa).The results were as follows.The CM-penetrating spermatozoa traveled a longer distance with better linear motility than spermatozoa in semen.Also, in comparison with spermatozoa obtained by density gradient centrifugation (DGC) and the swim-up method, the proportion of spermatozoa with normal morphology was higher, although, the proportion of spermatozoa with the head vacuole did not change.No DNA fragmentation was detected in the CM-penetrating spermatozoa.This method has several advantages.The technical procedure is simple and easy.Physical damage to spermatozoa is reduced because it does not require any centrifugation or washing procedure.A higher collection ratio of morphologically normal spermatozoon is achievable compared to the DGC and swim-up method, and it is a physiological selection method.We conclude that the CM penetration-based spermatozoa-sorting method is a promising new technique for ICSI because it is possible to collect physiologically better spermatozoa than those selected in the conventional selection method.
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