Biomimetic Natural Enzyme with Peptide-modified Gold Nanaoparticles

• 作 者: Xin Jiang Junrong Zhang Siqi Lin Yi Guo Li Xu
• 机 构: Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education,Jilin Universit
• 出 处: 第十一届中国酶工程学术研讨会
• 发表日期: 2017年7期
• 关键词: Nanotechnology Nanozyme Self-assembled monolayer (SAM) Glutathione peroxidase (G

　　Selenopeptide(Sec-Arg-Gly-Asp-Cys,URGDC)and cysteamine-cofunctionalized gold nanoparticles monolayer protected cluster(MPC),catalyzes the reaction of H202 by GSH,exhibiting Glutathione peroxidase(GPx)-like activity.The nanozyme was constructed through two self-assemble steps: First,URGDC was conjugated to AuNPs,forming a multivalent catalyst(MPC 1);second,the positively charged cysteamine was subsequently employed to optimize the enzyme activity by regulating the surface chemistry of gold nanoparticles(MPC 2).