【摘 要】
：
目的 研究青藤碱对变应性鼻炎的治疗作用并初步探究其相关机制.方法 采用4-6周BALB/c雄性小鼠进行实验研究.将小鼠分为三组,正常组,变应性鼻炎组及青藤碱组,每组十只.其中变应性鼻炎组和青藤碱组每只小鼠在第0,7,14天腹腔注射含25μg卵清蛋白和1mg氢氧化铝的PBS溶液1ml,正常组注射普通PBS溶液1ml.第21天开始,变应性鼻炎组和青藤碱组小鼠每天用含500μg卵清蛋白的PBS溶液滴鼻1
【机 构】
：
武汉大学中南医院 武汉大学人民医院
【出 处】
：
中华医学会2014年全国变态反应学术会议
论文部分内容阅读
目的 研究青藤碱对变应性鼻炎的治疗作用并初步探究其相关机制.方法 采用4-6周BALB/c雄性小鼠进行实验研究.将小鼠分为三组,正常组,变应性鼻炎组及青藤碱组,每组十只.其中变应性鼻炎组和青藤碱组每只小鼠在第0,7,14天腹腔注射含25μg卵清蛋白和1mg氢氧化铝的PBS溶液1ml,正常组注射普通PBS溶液1ml.第21天开始,变应性鼻炎组和青藤碱组小鼠每天用含500μg卵清蛋白的PBS溶液滴鼻1次,正常组小鼠则用普通PBS滴鼻,各组小鼠均连续滴鼻7天.青藤碱小鼠于滴鼻当天开始用0.5％的青藤碱喂养,其他组小鼠仍用普通饲料喂养.于最后一次滴鼻24小时后处死小鼠.各组小鼠取鼻部组织固定,HE染色;ELISA检测血清中特异性抗OVA-IgE,IL-4和IFN-Y浓度;Western Blot检测转录因子T-bet和GATA3表达水平.
其他文献
Background Effective treatment of patients with pelvic fracture during war or natural disasters is a worldwide problem.We aimed to investigate the effect of early internal iliac artery embolization fo
Background This study was to investigate the incidence of depression of coronary artery bypass grafting before and after surgery in patients with coronary heart disease and the impact of depression on
Background Using ultrasound biomicroscope (UBM) which have a high resolution to detecte radial artery wall structure have a great feasibility and high accuracy.In our study,we aim to explore clinical
Background Acute myocardial infarction (AMI) is one of the important causes that led to the injured person to death and disability following natural disasters.However,due to the limitations of inner s
Background Objective pathogenesis of acute myocardial infarction is coronary atherosclerosis(coronary arteries are also other diseases such as inflammation,spasms,deformity,etc) caused by continuous v
Background Percutaneous Coronary intervention (PCI) has by far,been a successful and effective method in treating coronary heart diseases.One of the most common complications with interventional cardi
Background Restenosis of percutaneous coronary intervention (PCI) often caused series of clinical symptoms that needed to implement revascularization.PCI patients through extended care can significant
Background The interventional examination and treatment of coronary heart disease (CHD) has been widely carried out in clinic,as a primary diagnosis of cardiovascular disease (CVD) method and the main
Background Due to the radial artery approach has advantages of min-invasive and quick recovery,it has been widely used in percutaneous coronary intervention.However,the proximal radial artery must be
Background Percutaneous coronary intervention (PCI) is the preferred method for the treatment of coronary heart disease ,not only because it has small trauma,but also it can restore blood flow of thos