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The aim of the study is to demonstrate positive influence of physical plasma in wound healing.Plasma is generated by adding energy to a gas resulting in ionization and excitation of gas molecules.Practical qualities of physical plasma are a result of different plasma components: electromagnetic radiation (UV, VIS, IR, high-frequency electromagnetic fields, etc.) on the one hand and ions, electrons and reactive chemical species, primarily radicals, on the other.Its use in medicine is a new and emerging field of research called plasma medicine.A prospective, randomized, controlled experimental procedure with blinded analysis compared intra-individually recovery of 4 similar small wounds, applied experimentally by CO2-laser (single shot of 20 watt, 100m J, 200 pulse/min) at each of 5 human subjects lower arms and then treated by various different patterns of plasma application (10 seconds/single short time, 30 seconds/single long time, 10 seconds at 3 following days/short time repeatedly, no treatment/control).The result of healing was decided by aspects such as the color and the texture of the recovering skin related to the surrounding untreated skin.Plasma was applied by the kINPen MED (INP Greifswald/neoplas GmbH, Greifswald, Germany).The device consists of a hand-held unit (dimensions: length=180 mm, diameter=20 mm, weight=170 g) for the generation of a gas discharge under atmospheric pressure conditions and a DC power supply unit (max.system power: 50 VA at 230 V, 50 Hz).In the centre of a ceramic capillary (inner diameter 1.6 mm) a pin-type electrode (1 mm diameter) is mounted.A high voltage of 2-3 kVpp at a frequency of 1 MHz is periodically (frep=2.5 kHz, plasma duty cycle=1∶1) applied to the pin electrode.As a result short time plasma stimulation, repeatedly applied for 3 days, looks to be the most effective wound treatment in terms of early recovery.In summary, 5 clinical case reports with identical treatment protocols support the idea, that physical plasma stimulation is positively influencing healing of superficial skin lesions.This is in line with previous results by Kalghatgi and colleagues reporting enhancement of cell proliferation in vitro by atmosphericpressure plasma treatment [Kalghatgi et al.2010].In a recent clinical trial, a tendency of faster closure of chronic ulcers after plasma treatment was found which was possibly caused not only by antiseptic plasma effects but also by additional stimulation of tissue regeneration [Heinlin et al.2011].