【摘 要】
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Although silica nanoparticles(SiNPs)have a promising application in biomedical fields,there is still a lack of comprehensive understand on genome-wide transcriptional analysis.This study is aimed to c
【机 构】
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Department of Toxicology and Sanitary Chemistry,School of Public Health,Capital Medical University,B
【出 处】
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首都医科大学公共卫生学院第六届研究生学术论坛
论文部分内容阅读
Although silica nanoparticles(SiNPs)have a promising application in biomedical fields,there is still a lack of comprehensive understand on genome-wide transcriptional analysis.This study is aimed to clarify the toxic effect and molecular mechanisms of SiNPs in zebrafish embryos based on microarray analysis and bioinformatics analysis.Microarray data analysis demonstrated that SiNPs-induced toxicity in zebrafish embryos affected expression of 2515 genes,including 1107 genes were up-regulated and 1408 genes were down-regulated.These differentially expressed genes were subjected to bioinformatic analysis for exploring biological processes triggered by SiNPs in zebrafish embryos.Gene ontology analysis showed that SiNPs caused significant changes in gene expression patterns related to many important functions,including response to stimuli,immune response,cellular process,and embryonic development.In addition,Pathway analysis and the Signal-net analysis indicated that the Gap junction,Vascular smooth muscle contraction,Metabolic pathways,Apoptosis,MAPK signaling pathway,Calcium signaling pathway and JAK-STAT signaling pathway were the most prominent significant pathways in SiNPs-induced toxicity in zebrafish embryos.In addition,results from qRT-PCR and western blot analysis showed that the IL-6 dependent JAK1/STAT3 signaling pathway was activated by SiNPs in zebrafish embryos.In summary,our data will provide compelling clues for further exploration of SiNPs-induced toxicity in zebrafish embryos.
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