【摘 要】
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Manganese(Mn)is an important environmental risk factor for Parkinsons disease (PD)and overexposure may result in manganism,a syndrome that closely resemble PD.Autophagy is an evolutionarily conserved
【机 构】
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Department of Occupational Health and Environmental Health,School of Public Health,Capital Medical U
【出 处】
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首都医科大学公共卫生学院第六届研究生学术论坛
论文部分内容阅读
Manganese(Mn)is an important environmental risk factor for Parkinsons disease (PD)and overexposure may result in manganism,a syndrome that closely resemble PD.Autophagy is an evolutionarily conserved process involving the removal of damaged proteins and organelles through a lysosomal-mediated pathway.The aim of this study was to determine whether SIRT1 mediated autophagy induced by manganese in SH-SY5Ycells.We investigated effects of MnCl2 at dose of 0,0.125,0.25,0.5 and 1mM on cells viability of SH-SY5Y cells by MTT.The autophagy-related proteins LC3 and Beclinl were detected by western-blotting.In addition,autophagy flux was monitored by fluorescence confocal microscope.The mRNA and protein expression levels of SIRT1 were also assessed.Further,we explored the role of SIRT1 in Mn-induced autophagy by using SIRT1-specific inhibitor Ex-527 and SIRT1-specific activator SRT1720.Our results suggested that MnCl2 inhibited cellular viability of SHSY5Ycells in a dose dependent manner.The autophagy and the formation of autophagic flux was obviously induced in MnCl2-treated SHSY5Y cells.Moreover,the expression of SIRT1 was activated at both mRNA and protein levels.Further studies revealed Mn-induced autophagy was activatied with pretreatment of SRT1720 wherase suppressed with pretreatment of Ex-527.In summary,we demonstrated that SIRT1-mediated autophagy induced by manganese in SH-SY5Y cells.
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