【摘 要】
：
Actin participates in many fundamental cellular processes,which take place not only in the cytoplasm,such as cell motility,cell division,and vesicle movement,bu
【机 构】
：
BiodynamicOpticalImagingCenter(BIOPIC),SchoolofLifeSciences,PekingUniversity,Beijing100871
【出 处】
：
中国化学会第三届全国生物物理化学会议暨国际华人生物物理化学发展论坛
论文部分内容阅读
Actin participates in many fundamental cellular processes,which take place not only in the cytoplasm,such as cell motility,cell division,and vesicle movement,but also in the nucleus,including chromatin remodeling,RNA processing,and transcription regulation.However,live cell imaging of nuclear actin with high spatial resolution remains to be a challenging task,partially due to the low concentration of nuclear actin compared to its cytoplasmic counterpart.Here we developed a method that combines LifeAct,a 17-amino-acid F-actin probe and multicolor Bimolecular Fluorescence Complementation (BiFC) to spectrally separate nuclear F-actin from cytoplasmic F-actin,achieving differential imaging of F-actin in distinct subcellular compartments with high signal-to-noise ratio.
其他文献
The outer membrane proteins (OMPs) are a kind of β-barrel proteins that are located on the outer membrane of Gram-negative bacteria.Precise understanding of th
Streptococcus mutans (S.mutans) is a common conditioned pathogen in human oral cavity.It is able to attach to the tooth surface and form biofilm,becoming a prim
Understanding how nanoparticles interact with pulmonary surfactant monolayer (PSM) is quite important for safe biomedical applications of nanoparticles and
Appropriate interaction between the T-cell receptor (TCR) and peptide-major histocompatibility complex (pMHC) initiates adaptive immune responses,but the mechan
The mechanical properties of films are of great importance for their use as biocompatible surface coatings or for drug encapsulation and release1~4.In this
线粒体是细胞代谢过程扮演重要角色的奇妙细胞器,同时也是一个半自主性细胞器.近年来的研究发现:线粒体是许多药物和毒物作用的靶细胞器,线粒体重新成为研究热点.有科学家用"
In 2005 we discovered that previously-thought insoluble proteins could in fact be solubilized in unsalted water.Since then,this discovery has offered us and oth
Simulating biologically relevant timescales at atomic resolution is a challenging task since typical atomistic simulations are at least two orders of magnit
Our equilibrium molecular dynamics (MD) simulations for aqueous sodium chloride solutions revealed that,supplementary to the Debye-H(u)ickel theory,reversible t
Getting rid of ensemble average,experiments on single molecule level are informative and powerful to study the dynamics of biomolecutar system.In order to leam