论文部分内容阅读
The potential lead compound of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) inhibitor was studied by a combination of high-performance affinity chromatography (HPAC) and antisense peptide based combinatorial peptide libraries.A functional and highly conserved aromatic-aa-rich peptide firagment within the transmembrane domain of spike protein was chosen as sense peptide (SP) [1].Four antisense peptides (AP1-AP4) were designed based on the principle of complementary base pairs and the degeneracy of genetic code [2].The interactions between SP and its antisense peptides were studied by the HPAC system with SP as the affinity ligand, pH 5.5 was chosen in the screening to mimic the pH environment required by virus fusion [3].The Arg-rich peptide AP4 (RNIRRPG) exhibited the strongest affinity binding to immobilized SP.