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The population of asparagus has high heterogeneity because of its dioecious nature, and it is difficult and time consuming to produce inbred lines.Production of doubled haploid lines is actually commonly used by plant breeders in order to obtain pure lines in the shortest possible time.In this study, production of haploid plants was attempted by means of polyembryonic seeds, and origin of polyembyonic seeds was also investigated through SSR and flow cytometric analyses.Seeds obtained from eighteen combinations of crosses were provided.The frequency of seed germination was 92.9% (2,718 seeds germinated out of 2,925 seeds sown).