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Hydrophobic interaction chromatography (HIC) is one of the widely used methods for protein separation and purification, in which hydrophobicity of chromatographic media is a very important factor in theory and practice.The hydrophobicity of a traditional HIC stationary phase usually would not change much after produced.Therefore, different HIC media is often tested to find one with suitable hydrophobicity for separation of a given protein sample.However, types of commercial available HIC media are very limited, thus the hydrophobicity variation is constrained in a very limited range, and continuous change of the hydrophobicity is also impossible.