One-pot Labeling-based Capillary Zone Electrophoresis for Separation of Amino Acid Mixture

• 作 者: Lijuan Song Yi Chen
• 机 构: Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academ
• 出 处: 第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
• 发表日期: 2011年3期
• 关键词: One-pot labeling Amino acids Capillary electrophoresis

　　Amino acids (AAs) are very important in the fields of biology and biochemistry.As the building blocks of proteins and bioactive composition in various biosystems, they are commonly resource-limited and have urged the development of microanalytical techniques.Capillary electrophoresis (CE) is such a type of micro technique that has high efficiency, high speed, low cost of operation and low consumption of samples.It is hence becoming a powerful tool in biomedical and clinical researches and in forensic practice.However, a great challenge appears in the analysis of AAs because they lack intrinsic groups such as chromophores for UV absorbance-or fluorescence-based detection.Various labeling methods have then been developed but most of them are complicated to operate.