聚乙烯亚胺与双链DNA相互作用的单分子力谱研究

来源 :中国化学会第三届全国生物物理化学会议暨国际华人生物物理化学发展论坛 | 被引量 : 0次 | 上传用户：nankaizhizhuan

【摘要】

：

数十年来,通过基因疗法治愈遗传疾病始终是人类矢志不渝的梦想.寻找合适的载体将外源基因送入细胞便是该疗法的核心.因此阳离子聚合物应运而生,它易于与DNA结合,而且细胞毒性

【作者】

：

寇晓龙张薇张文科

【机构】

：

吉林大学化学学院,130012,吉林长春

【出处】

：

中国化学会第三届全国生物物理化学会议暨国际华人生物物理化学发展论坛

【发表日期】

：

2014年7期

【关键词】

：

聚乙烯亚胺(PEI)-DNA 原子力显微镜(AFM) 单分子力谱

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数十年来,通过基因疗法治愈遗传疾病始终是人类矢志不渝的梦想.寻找合适的载体将外源基因送入细胞便是该疗法的核心.因此阳离子聚合物应运而生,它易于与DNA结合,而且细胞毒性低;转染效率高[1].其中聚乙烯亚胺(PEI)自1995年被首次使用以来,被认为是最高效的聚合物材料之一,其易制备、易改性、低成本等优点使之倍受青睐[2].从分子水平揭示PEI与DNA的作用机理对于优化载体设计具有重要意义.

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聚乙烯亚胺与双链DNA相互作用的单分子力谱研究

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