【摘 要】
：
Introduction Like other arteriviruses,PRRSV 5 UTR is believed to carry a variety of cis-acting elements that regulate the discrete steps of viral life cycle.But the regulatory function and mechanism n
【机 构】
：
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences,Shanghai 200241, Ch
【出 处】
：
2013国际猪繁殖与呼吸综合征学术研讨会
论文部分内容阅读
Introduction Like other arteriviruses,PRRSV 5 UTR is believed to carry a variety of cis-acting elements that regulate the discrete steps of viral life cycle.But the regulatory function and mechanism need to be addressed.PRRSV has been characterized into two distinct genotypes,type 1 and type 2.After alignment,the 5 UTRs of two genotypes are different in length and just share about 60％ nucleotide identity.Nevertheless,the secondary structures of 5 UTR in two genotypes are quite similar and characterized by six major putative stem-loops,especially the prominent one which transcription-regulatory sequence (TRS) located in.Our previous research showed that the prominent stem-loop structure in SL5 is very important for viral infectivity and RNA synthesis,but the detailed regulatory function of each part of the structure is lack of experimental research.According to RNA structure model,we arbitrarily divide the prominent stem-loop structure in SL5 into three portions A/A loop part,B/B stem part,C (common) top stem-loop.In this study,we focused on the stem part B/B in order to dissect its regulatory function in viral life cycle.
其他文献
The porcine reproductive and respiratory syndrome virus (PRRSV) ORFla-encoded replicase polyproteins (ppla) is predicted to be proteolytically processed into ten nonstructural proteins (nsps),known as
Background:Porcine circovirus type 2 (PCV2) is associated with post-weaning multisystemic wasting syndrome (PMWS) in pigs.Currently,there is considerable interest in the immunology of PCV2,in particul
Background:The conserved motif 97RIRKVKV103 in the porcine circovirus type 2 (PCV2) capsid protein (Cap) was initially presumed to be a binding site for cell surface proteoglycans.As this motif later
The generation of chimeric viruses,typically by replacing a gene sequence with the similar sequence from a related virus,has been a useful genetic tool in various virus systems to analyze infectivity,
Porcine circovirus type 2 (PCV2) is an important causative agent of postweaning multisystemic syndrome in pigs,and both replicase proteins (Rep and Rep) encoded by PCV2-ORF1 are essential for the vira
Porcine circovirus type 2 (PCV2) is generally considered to be the primary causative agent of postweaning multisystemic wasting syndrome (PMWS),which has become a serious economic problem for the swin
Background:Porcine circovirus type 2 (PCV2) is associated with postweaning multisystemic wasting syndrome in pigs.A monoclonal antibody (mAb) 8E4 againstthe PCV2 capsid protein has the capacity to neu
Background:Porcine reproductive and respiratory syndrome (PRRS) is an economically important disease,which leads to severe economic losses in swine-producing areas of the world.PRRSV ORF7 encodes the
Elucidating protein-protein interactions between viral and host cellular proteins is critical to understanding viral pathogenesis.A yeast two-hybrid screen revealed that both major PRRSV envelope prot
Main objective:Study the effect of a pre-viral infection with porcine reproductive and respiratory syndrome virus (PRRSV) on a bacterial infection by Actinobacillus pleuropneumoniae (App) in vitro.Spe