【摘 要】
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Main objective:Study the effect of a pre-viral infection with porcine reproductive and respiratory syndrome virus (PRRSV) on a bacterial infection by Actinobacillus pleuropneumoniae (App) in vitro.Spe
【机 构】
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Groupe de recherche sur les maladies infectieuses du porc(GREMIP), Centre de recherche en infectiolo
【出 处】
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2013国际猪繁殖与呼吸综合征学术研讨会
论文部分内容阅读
Main objective:Study the effect of a pre-viral infection with porcine reproductive and respiratory syndrome virus (PRRSV) on a bacterial infection by Actinobacillus pleuropneumoniae (App) in vitro.Specific objectives:Compare adherence between mutant strain of App AapxICAapxⅡIC (AppA),producing non active ApxI and ApxⅡI toxins,and wild type App (AppWT) strain in PRRSV co-infected SJPL cells.-Compare toxicity of AppA and AppWT strain in PRRSV co-infected SJPL cells.-Study the impact of AppA and PRRSV co-infection in different cell types,SJPL,MARC-145 and PAMs.Brief methods:SJPL cells were infected with PRRSV and co-infected with AppA or App WT,then adherence was measured (CFU/well) and cell cytotoxicity was measured using lactate dehydrogenase (LDH) assay.For PRRSV and AppA co-infections;SJPL,MARC-145 and PAM cells were infected with PRRSV and co-infected with AppA,UV inactivated AppA or LPS,then immunofluorescence assay (IFA) detection of PRRSV N viral protein and PRRSV titration on MARC-145 cells using the K～ber method was performed.
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