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AIM To identify salvianolic acid L (SAL) metabolites in rats.METHODS Bile duct of wistar rats (n =6, male)was cannulated with PE tubing after anesthesia.Then bile samples were collected for 24 h after intravenous injection of 20 mg· kg-1SAL.Another group of 6 male Wistar rats were kept in metabolism cage after intravenous injection of 20 mg·kg-1 SAL, urine samples were collected for 24 h and blood samples were drawn from ophthalmic veins of the rats by sterile capillary tube.The samples were acidized by HCl 1 mol· L-1.Then extraction of SAL and its metabolites were conducted with ethyl acetate.After removal of organic solvent by nitrogen gas blowing, samples were reconstituted in mobile phase solvent before subjected to liquid chromatography-mass spectrometry analyses.RESULTS About 60% of SAL and its metabolites was excreted from bile.Six monomethylation metabolites,four dimethylation metabolites, 1 monoglucturonidation metabolites, monoglucuronidation-monmethylation metabolites and monoglucuronidation-dimethylation metabolites were discovered in bile of wistar rats, and the metabolites were also detected in plasma and urine.CONCLUSION The main metabolic pathway of SAL in Wistar rats was methylation and glucuronidation.Bile excretion was the main excretion pathway, indicating that liver played a very important role in the metabolism of SAL.