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Cyclodextrin electrokinetic chromatography (CDEKC) is one of the versatile methods for the enantioseparation in capillary electrophoresis.CDEKC has great advantages such as the rapid separation, high resolution and low consumption of samples/reagents, while a low concentration sensitivity due to a short optical path length is often problematic.To improve the sensitivity, various on-line and off-line preconcentration techniques have been applied to CDEKC [1].In this study, a novel on-line sample preconcentration technique named transient trapping (tr-trapping) [2] was combined with CDEKC to achieve a rapid and highly sensitive enantioseparation.