【摘 要】
：
On-line concentration via Electrokinetic Supercharging (EKS) was used to enhance the sensitivity of the capillary electrophoretic separation of the four flavonoids narigenin, hesperetin, naringin and
【机 构】
：
Institute of Materia Medica, Shandong Academy of Medical Sciences, Jinan 250062, China;Analysis and
【出 处】
：
第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
论文部分内容阅读
On-line concentration via Electrokinetic Supercharging (EKS) was used to enhance the sensitivity of the capillary electrophoretic separation of the four flavonoids narigenin, hesperetin, naringin and hesperidin.Hexadimethrine bromide(HDMB) was used to coat the capillary for electroosmotic flow(EOF) reversal.Separtion condisions, including the background electrolyte pH and concentration, the length and choice of terminator and the electrokinetic injection time were optimized.The optimum conditions were: a background electrolyte of 30 mM sodium tetraborate (pH 9.5) containing 5% (v/v) of methanol, electrokinetic injection of the sample (130 s,-10 kV) followed by hydrodynamic injecting of 100 mM 2-(cyclohexylamino)ethanesulphonic acid (CHES) (17 s, 0.5 psi) as terminator, and separation with-20 kV.Under these conditions the four flavonoids could be separated with a sample-to-sample time of 15 min and detection limits from 1.0 to 3.4 ng/mL.
其他文献
In this study, we developed a nanoliter-scale droplet array to perform digital PCR for absolute gene quantification.The droplet array was automatically generated on a microchip with a droplet dispense
Gold nanoparticles (GNPs) have been widely used as one of the most versatile nanomaterials in a variety of fields such as catalysis, sensors and biomedicine, due to their unique physico-chemical prope
Quantitative protein analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in the selected reaction monitoring (SRM) mode is developing into a major alternative to current immunochemic
High-performance separation of ultrasmall volume sample is crucial to modern biochemical analysis and microfluidics has significantly contributed to the miniaturization and improvement of separation e
Fuel gas is a general term for any of gas burned to produce thermal energy, including natural gas, liquefied petroleum gas, coal gas, and biogas and so on.For the impact of sources and their environme
Quantum dots (QDs), with their superior size-dependent fluorescence properties, have been employed as non-covalent fluorescent labels for the determination of tomato systemin (TomSys) by capillary ele
Flavonoids occur as a group of active constutents in many traiditional Chinese medicines, so to analyze them accurately is of much importance.With the advantages of low consumption of reagents and sam
In this paper, a MIP coating grafted to a trimethylolpropane trimethacrylate (TRIM) core material in a capillary for electrochromatography (CEC) chiral separation of amlodipine was reported.A core mon
Adsorption of proteins onto the capillary wall leads to poor performance when capillary electrophoresis frontal analysis (CE-FA) is used for determination of drug-protein binding parameters.Herein, we
Gene mutation detection is playing an important role in clinical disease diagnosis.Now, gene mutation always detected by traditional methods as polyacrylamide gel basis on polymerase chain reaction.Bu