【摘 要】
：
Human cytochrome P450 1A (CYP1A), one of the most important phase Ⅰ important drug-metabolizing enzymes in human, plays a significant role in metabolism of therapeutic drugs and activation of environm
【机 构】
：
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
【出 处】
：
2015年第一届药代动力学朝阳论坛
论文部分内容阅读
Human cytochrome P450 1A (CYP1A), one of the most important phase Ⅰ important drug-metabolizing enzymes in human, plays a significant role in metabolism of therapeutic drugs and activation of environmental contaminants or other xenobiotics to toxic or carcinogenic species.By far, most of CYP1A fluorescent probes were intensity-based and work with one photo microscopy, which may be disturbed in quantitative detection by many factors.Therefore, a more simple, sensitive and practicable method based on fluorescence probe for CYP1A is highly desirable.Hererin, a ratiometric two-photon fluorescent probe (NCMN) for the selective and sensitive detection of CYP1A has been designed, synthesized and fully evaluated.NCMN can be selectively dealkylated by CYP1A, accompanied with the remarkable changes in absorption and fluorescence spectra (red-shifted emission 114 nm), allowing the naked-eye visible and fluorescence analysis.
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