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Aim: The concentration of the multifunctional extracellular matrix glycoprotein,osteopontin (OPN) is increased in inflammatory tissues and is believed to modulate the functions of inflammatory cells.In our study, the effects of OPN on inflammatory responses of human mast ceils are investigated.Methods: OPN extracted from human milk and human mast cells cultured from CD34+ monocytes isolated from peripheral blood were employed in the current stud y.Mast cell activation was induced by anti-IgE.