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To dissect the genetic basis of oil content in B.napus is important for seed oil content improvement in breeding of rapeseed (B.napus L.).A large and new KN DH population containing 348 lines was obtained from a cross between KenC-8 and N53-2 with oil content difference over 10%in seed oil content.The KN DH population was phenotyped in eight natural environments and subjected to quantitative trait loci (QTL) analysis for oil content.