Solvent exposure analysis of glucagon with 19F nuclear magnetic resonance

来源 :上海浦东免疫化学国际生物论坛 | 被引量 : 0次 | 上传用户：sdhok

【摘要】

：

Fluorine-19 nuclear magnetic resonance (19F NMR) spectroscopy is a powerful method in studying the conformation, topology and interactions of biological molecul

【作者】

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Yaguang Hou Wanhui Hu Xiaona Li Dongsheng Liu Kurt Wüthrich

【机构】

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iHumanInstitute,ShanghaiTechUniversity

【出处】

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上海浦东免疫化学国际生物论坛

【发表日期】

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2015年11期

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Fluorine-19 nuclear magnetic resonance (19F NMR) spectroscopy is a powerful method in studying the conformation, topology and interactions of biological molecules.Here, we applied a 19F-containing amino acid (4-fluoro-L-phenylalanine, 4F-Phe) to label glucagon.With a standard curve for 4F-Phe chemical shifts for varying D20 concentrations, site-specific 19F solvent isotope shifts were analyzed for glucagon to compare solvent exposure of this peptide.

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Solvent exposure analysis of glucagon with 19F nuclear magnetic resonance

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