Analysis of calcium dynamics using Yellow Cameleon 3.6 indicator driven by UBQ10 promoter in rice

来源 :第十五届全国植物基因组学大会 | 被引量 : 0次 | 上传用户：jh_viso1

【摘要】

：

Calcium plays an important role in higher-plant signaling pathways.Yellow cameleon(YC)is a powerful tool to monitor the spatial-temporal dynamics of Ca2+ fluxes

【作者】

：

Nili Wang Smrutisanjita Behera Chunxia Zhang Lizhong Xiong J(o)rg Kudla

【机构】

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NationalKeyLaboratoryofCropGeneticImprovementandNationalCenterofPlantGeneResearch(Wuhan),HuazhongAgr

【出处】

：

第十五届全国植物基因组学大会

【发表日期】

：

2014年9期

【关键词】

：

calcium YCs Ca2+-sensor imaging cameleon UBQ10 Oryza sativa

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Calcium plays an important role in higher-plant signaling pathways.Yellow cameleon(YC)is a powerful tool to monitor the spatial-temporal dynamics of Ca2+ fluxes.Here,we for the first time generated rice transgenic lines that contain Yellow cameleon 3.6(YC3.6)indicator.We used the Arabidopsis UBIQUITIN10(UBQ10)promoter in addition to the routine Cauliflower mosaic virus(CaMV)35S promoter because the 35S promoter may cause gene silencing and co-suppression events.

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Analysis of calcium dynamics using Yellow Cameleon 3.6 indicator driven by UBQ10 promoter in rice

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