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Sex hormone test provides an important reference value and basis for the clinical diagnosis of infertility.It also provides guidance for clinicians to understand the etiology and treatment of patients.Follicle-stimulating hormone(FSH)is a kind of glycoprotein gonadotropins,which is an important inspection index in clinical detection of female ovarian diseases.The concentration level of FSH is important for the evaluation of female reproductive health.Paper-based microfluidic sensor is a kind of biochemical analytical technology with wide application foreground.