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The aim of this work was to utilize sweet potato for production of bioethanol.Optimal conditions of α-amylase and glucoamylase was carried on hydrolyzation of sweet potato and measurement of reducing sugars.The optimum α-amylase concentration and volume were 0.05% (w/v) and 5 ml, respectively at 90℃ for 2 h.These conditions, the maximum concentration of reducing sugar was 16.43 gL-1.The optimum glucoamylase concentration and volume were 0.015 (w/v) and 20 ml, repectively at 60℃ for 4 h.The concentration of reducing sugar was 41.78 gL-1.After hydrolyzation of sweet potato with these two enzymes at optimal condition and fermented using Saccharomyces cerevisiae YRK 017 (isolated form Loog-pong).The maximum ethanol concentration of 14.55 gL-1 was achieved after 72 h of separate hydrolysis and fermentation process (SHF).For simultaneous saccharification and fermentation process (SSF), the maximum concentration of ethanol was 12.62 gL-1.