【摘 要】
：
Senescence is an important obstacle to cancer.To investigate the effects of phosphorylation Liver Kinase B1(pLKB1) on the stemness maintaining of acute myeloid leukemia(AML) stem cells,we determine th
【机 构】
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Institute of Hematology,Wuhan Union Hospital,Huazhong University of Science and Technology
【出 处】
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The 35th World Congress of the International Society of Hema
论文部分内容阅读
Senescence is an important obstacle to cancer.To investigate the effects of phosphorylation Liver Kinase B1(pLKB1) on the stemness maintaining of acute myeloid leukemia(AML) stem cells,we determine the expression level and effect of pLKB1 on senescence of CD34+CD38- KG1acells containing rich AML stem cells.The proteins of pLKB1 and SIRT1 (Sirtuin 1),a regulator of pLKB1,were detected with western blotting.Senescence associated markers were separately tested by real time PCR (for p21 mRNA),SA-β-gal staining,MTT Assay(for cell proliferation),flow cytometry (for Cell Cycle Assay).Besides,apoptosis was assayed by flow cytometry.
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