【摘 要】
：
The somata of primary sensory neurons,including dorsal root ganglion(DRG)neurons,release neurotransmitters and neuropeptides.Following physiological action potentials,in addition to Ca2+-dependent sec
【机 构】
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State Key Laboratory of Membrane Biology,Institute of Molecular Medicine,Peking University,Beijing 1
【出 处】
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第九届海内外华人神经科学家研讨会(The 9th Symposium for Chinese Neuroscientis
论文部分内容阅读
The somata of primary sensory neurons,including dorsal root ganglion(DRG)neurons,release neurotransmitters and neuropeptides.Following physiological action potentials,in addition to Ca2+-dependent secretion,we have discovered and studied Ca2+-independent but voltagedependent secretion(CiVDS)in somata of freshly isolated DRG neurons(Zhang et al,2002,2004; Zheng et al,2009,Liu et al,2011).Major open question of CiVDS is the molecular mechanism,including 3 components: fusion pore machinery(FP),voltage sensor(VS)and the FP-VS linker(LK).Here we report,by using exocytosis assays of patch-clamp recording of membrane capacitance,and single vesicle imaging(EM and TIRF),(1)FP is jointly contributed by 2 components of SNARE complex,SNAP25 and syntaxin;(2)VS is contributed by voltage-gated Ca2+channels(VGCCs),Cav2.2(N-type VGCC);(3)LK is the “synprint”,Cav2.2 intracellular loop 718-963aa(Catterall,1999);(4)following automatic knockdown of CiVDS by 3d-culture of DRG neurons,CiVDS is rescued by overexpressing any component of FP(SNAPE25 or syntaxin)or VS(Cav2.2);(5)CiVDS is inhibited by blockers against FP(SNAP25 or syntaxin),VS(Cav2.2)and LK(synprint-truncated);(6)finally,CiVDS is blocked by Cav2.2-RNAi-KD in DRG pretransfected in vivo.
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