【摘 要】
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More and more efforts are endeavored in the ultra-sensitive quantification of peptides and proteins, because many important peptides and proteins are present at ultra-low levels and only the quantity
【机 构】
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Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial
【出 处】
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第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
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More and more efforts are endeavored in the ultra-sensitive quantification of peptides and proteins, because many important peptides and proteins are present at ultra-low levels and only the quantity of them or the changes in their abundance really reflects the status and changes of a biological system.We herein reported a novel ultra-sensitive method for the simultaneous quantification of three β-casomorphins based on the Eu3+ diethylenetriamine-N,N,N,N",N"-pentaacetic acid labeling and capillary electrophoresis with on-line inductively coupled plasma mass spectrometry detection (CE-ICP-MS).The europium-tagged β-casomorphins were effectively separated by capillary electrophoresis and were sensitively determined by inductively coupled plasma mass spectrometry.
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