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  5. A Simple Interface and Method for Online Coupling Solid Phase Extraction with High Performance Liqui

A Simple Interface and Method for Online Coupling Solid Phase Extraction with High Performance Liqui

来源 :第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会 | 被引量 : 0次 | 上传用户：ssddhwl
【摘 要】
：
Trace analysis is popular in food, environmental and biological sample.For the high resolution and especially suitable for polar, unvolatile and thermal unstable compounds, high performance liquid chr
【作 者】
：
Guilan Huang Jin-Ming Lin Haifang Li 
【机 构】
：
Chemistry Department, Tsinghua University, Beijing, 100084, China
【出 处】
：
第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
【发表日期】
：
2011年3期
【关键词】
：
Interface Online SPE-HPLC Trace analysis 
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　　Trace analysis is popular in food, environmental and biological sample.For the high resolution and especially suitable for polar, unvolatile and thermal unstable compounds, high performance liquid chromatography (HPLC) based techniques are preferred to analyze more than 70% chemicals.Solid phase extraction (SPE) is the most effective and convenient sample pretreatment method in trace analysis until now.However, offiine SPE is usually laborious and time consuming.As an alternative method, online SPE-LC has been developed to improve the sensitivity with a relative low sample volume and labor-saving.Most reported online SPE-LC were set up with multiposition valve and additional pumps for sample loading and using the guard column as the SPE to trap analytes.It makes the construction of online system is high cost and the selection of SPE is limited.The other choice is using one of the HPLC pumps to load sample on the base of on-column focusing technique.It is simple and economic, but usually not so effective because of the serious extra-column effect.
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