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A fast, simple method for the determination of nicotine and its metabolites in human saliva was developed and validated in this paper.Human saliva was collected by Salivette(R) collection kit and the quantification was achieved by using nicotinemethyl-d3, cotinine-methyl-d3 and trans-3-hydroxycotinine-methyl-d3 as internal standard under atmosphere pressure chemical ionization(APCi) mode of liquid chromatography-tandem mass spectrometry(LC-MS/MS).The pretreatment of this method was simple, fast and all analytes were eluted within lmin.The relative standard deviation(RSD%) of nicotine and its two metabolites were between 0% and 4.03% with the recovery rate ranged from 93.9% to 104.2%.The linear correlations were above 0.995.Saliva,plasma and urine samples from 70 volunteers were collected and quantified by the described procedure.The classification of different smoking amount was also made using ada-boost based on the total concentration of salivary nicotine and its metabolites with the predicting accuracy of 76%.A good correlation of cotinine levels bewteen saliva and plasma(r=0.92),saliva and urine(r=0.84) was found.The results showed that the cotinine in saliva has a longer half-life and was the most desirable biomarker for the evaluation of smoking exposure and nicotine uptake.