【摘 要】
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Given the tremendous growth of environmental and occupational exposures, concerns about the adverse health effect of benzene have been raised.Our study aimed to explore the newly sensitive biomarker o
【机 构】
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Department of Occupational Health and Environmental Health, School of Public Health, Capital Medical
【出 处】
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2016(第二届)毒性测试替代方法与转化毒理学(国际)学术研讨会暨有害结局路径(AOP)与风险评估培训会议
论文部分内容阅读
Given the tremendous growth of environmental and occupational exposures, concerns about the adverse health effect of benzene have been raised.Our study aimed to explore the newly sensitive biomarker of adverse health effect for benzene exposure and the toxic mechanisms of benzene metabolite in vitro.This study contained 314 benzene-exposed workers and 288 controls that were exposed to the air benzene concentration of 2.64±1.60 mg/m3 and 0.05±0.01mg/m3, respectively.Compared to controls, miR-34a expression was higher in benzene-exposed workers than controls.Correlation analysis showed that miR-34a was obviously associated with blood cell counts, Alanine transaminase (ALT) and oxidative stress.Further, the multivariate analysis showed that the expression of miR-34a was the most strongly correlated with white blood cell (structure loadings=0.952).Furtherly the underlying mechanism of miR-34a on 1,4-Benzoquinone-induced apoptosis were performed using lentivirus vectors transfection.The results showed that 1,4-Benzoquinone induced apoptosis and simultaneously upregulated miR-34a accompanying with the decrease of Bcl-2.Inhibition of miR-34a elevated Bcl-2 and decreased 1,4-Benzoquinone-induced apoptosis.In conclusion, miR-34a was involved in benzene-induced hematotoxiticy by targeting Bcl-2 and could be regarded as a newly potential biomarker for benzene toxicity.
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