【摘 要】
：
It has been proved that TRAM-derived decoy peptides have anti-inflammatory properties.In this study,we synthesized a TRAM-derived decoy peptide (TM6), belongs to TRAM TIR domain, of which sequence is
【机 构】
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Department of Clinical Veterinary Medicine,College of Veterinary Medicine, Jilin University,Changchu
【出 处】
：
中国畜牧兽医学会兽医内科与临床诊疗学分会第八届代表大会暨学术研讨会
论文部分内容阅读
It has been proved that TRAM-derived decoy peptides have anti-inflammatory properties.In this study,we synthesized a TRAM-derived decoy peptide (TM6), belongs to TRAM TIR domain, of which sequence is "N"-RQIKIWFQNRRMKWK, KENFLRDTWCNFQFY-"C" and evaluated the effects of TM6 on lipopolysaccharide-induced mastitis in mice.In vivo, LPS-induced mice mastitis model was established by injection of LPS through the duct of mammary gland.TM6 was injected 1 h before or after LPS treatment.In vitro, primary mouse mammary epithelial cells were used to investigate the effects of TM6 on LPS-induced inflammatory responses.The results showed that TM6 inhibited LPS-induced mammary gland histopathologic changes, MPO activity, and TNF-α, IL-1β and IL-6 production in mice.In vitro, TM6 significantly inhibited LPS-induced TNF-α and IL-6 production,as well as NF-κB and MAPKs activation.In conclusion, this study demonstrated that TM6 had protective effects on LPS-mastitis and may be a promising therapeutic reagent for mastitis treatment.
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