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Dairy cows are often fed a high-concentrate diet to meet lactating demands, yet long-term concentrate feeding induces subacuteruminal acidosis (SARA) and leads to a decrease in milk fat.The liver is responsible for lipid metabolism in ruminant animals, and SARA is associated with liver abscesses, fatty liver and a whole-body inflammatory response when a high-grain diet is offered.Stearoyl-CoA desaturase1 (SCD1) participates in fatty acid biosynthesis in the liver of lactating ruminants.Therefore, the consequence of SARA is bound to influence the milk fat precursors of which synthesized in the liver.