【摘 要】
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[Background/Aims]An elevated concentration of plasma non-esterified fatty acids (NEFA) is an important indicator of negative energy balance (NEB), which is often associated with metabolic diseases suc
【机 构】
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College of Veterinary Medicine, Northwest A & F University,Yangling 712100, P.R.China
【出 处】
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中国畜牧兽医学会兽医内科与临床诊疗学分会第八届代表大会暨学术研讨会
论文部分内容阅读
[Background/Aims]An elevated concentration of plasma non-esterified fatty acids (NEFA) is an important indicator of negative energy balance (NEB), which is often associated with metabolic diseases such as fatty liver and ketosis.Angiopoietin-like protein 4 (ANGPTL4) is a hepatokine that plays important roles in the regulation of lipid metabolism.However, the direct effects of high concentrations of NEFA on expression of ANGPTL4 and secretion in hepatocytes from cows are not entirely clear.Accordingly, the objective of this study was to investigate the effects of NEFA with different concentrations on expression of ANGPTL4 and secretion in calf hepatocytes cultured in vitro.[Methods]After the cells were incubated for 72 h, NEFA was added to the culture solution at final concentrations of 0.6, 1.2, 1.8 and 2.4 mmol/L.Culture supernatants and cells were collected after continuous culture for 24 h, and mRNA and protein expression levels of ANGPTL4 in the hepatocytes were detected by real-time polymerase chain reaction (real-time PCR) and western blot, respectively.The secretion of ANGPTL4 in the supernatant was detected by enzyme-linked immunosorbent assay (ELISA).[Results]The results showed similar expression and secretion of ANGPTL4 following treatment with 0.6 and 1.2 mmol/L NEFA, which were higher than those observed for the controls (P<0.05).The expression and secretion of ANGPTL4 were also similar at 1.8 and 2.4 mmol/L NEFA and significantly higher than those observed for controls (P<0.01).[Conclusions]These data suggest that high concentrations of NEFA significantly promote expression and secretion of ANGPTL4 in bovine hepatocytes.In particular, this promotion occurs in a dose-dependent manner and may be involved in the pathological processes of energy metabolism disorders of dairy cows in the peripartum period.
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