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[Objective] This paper aimed to provide references for pet dog kidney and urinary track diseases x-ray radiography diagnosis.[Method] Experimental dogs were gave two kinds of hypotonic medicines (Vitamin K3 and anisodamine) in advance, non-compression hypotonic (IVP) was done and radiography effects of this two hypotonic medicines were compared later in order to finally discuss clinical practical application value of canine (IVP) using hypotonic medicines.[Result] 3.5 mg/kg.bw anisodamine dosage was injected before radiography, 10 minutes after radiography was the best time to take photo, and the development of experimental dogs bilateral renal contour, renal pelvis and front segment of ureter was basically clear.While regarding radiography by 1.6 mg/kg.bw vitamin K3, the best photo was got 10 minutes later after radiography, and the development of experimental dogs bilateral renal contour, renal pelvis and front segment of ureter was clear.[Conclusion] Vitamin K3, as a kind of hypotonic medicine used in pet clinical (IVP), had characteristics of safety, low dosage and better practical radiography effect, was obviously better than anisodamine, and could be properly promoted in pet clinical (IVP).