【摘 要】
：
Telomeric G-quadruplex (G4) DNA structures play a critical role in regulating chromosome stability by inhibiting the telomerase activity.Here we report three folded states with markedly different mech
【机 构】
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Mechanobiology Institute,NUS,Singapore School of
【出 处】
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8th IUPAP International Conference on Biological Physics(第八届
论文部分内容阅读
Telomeric G-quadruplex (G4) DNA structures play a critical role in regulating chromosome stability by inhibiting the telomerase activity.Here we report three folded states with markedly different mechanical stability formed on DNA containing telomeric G4 sequence in physiologically relevant K+ concentrations.Our results suggest that predominant G4 folding is through a short-lived intermediate state to a longer-lived state, and the force-dependent transition free energy landscape is determined.In addition, a new form of telomeric G4 structure with a much stronger mechanical stability is identified.This work provides the first direct single-molecule measurements that characterize the equilibrium transitions of multiple conformers of telomeric G4 at physiological K+ concentration.
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