Comparative proteomic analysis of different Toxoplasma gondii genotypes by two:dimensional fluoresce

• 作 者: 周东辉 赵付荣 Alasdair J.Nisbet 徐民俊 宋慧群 黄思扬 朱兴全
• 机 构: 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点点实验室甘肃省动物寄生虫病重点验实验室,兰州 730046
• 出 处: 中国畜牧兽医学会家畜寄生虫学分会第七次代表大会暨第十二次学术研讨会
• 发表日期: 2013年7期

　　Toxoplasma gondii is a protozoan parasite infecting almost all warm:blooded animals and humans.There are three infective stages ofT.gondii: the tachyzoites,the bradyzoites and the oocysts.The tachyzoite is a rapidly multiplying stage and the main pathogenic factor.In North America and Europe,T.gondii is consisted of four major elonal lineages (namely Types Ⅰ,Ⅱ,Ⅲ and Type 12).In this study,we explored the proteomic profiles of different genotypes (Type Ⅰ:RH strain,Type Ⅱ:PRU strain,Type Ⅱ:TgQHO strain and ToxoDB 9:TgC7 strain) ofT.gondii tachyzoites by using two:dimensional fluorescence difference gel electrophoresis (2D DIG E) combined with MALDI:TOF MS.Totally,110 differentially abundant protein spots were selected.Of these,98 spots corresponding to 56 proteins from T.gondii were successfully identified.These included surface antigen (SAG1),heat shock protein 70 (Hsp70),disulfide isomerase,coronin,heat shock protein 60 (Hsp60),pyruva te kinase,receptor for activated C kinase 1 and peroxiredoxin.Gene ontology (GO) enrichment analysis reveale d that most of the differentially abundant proteins were involved in biological regulation,metabolic process,response to stress,binding,antioxidant activity and transporter activity.According to the Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathway maps ofT.gondii,some identified proteins were involved in the glycolytic/gluconeogenesis pathway.The present study identified differentially abundant proteins among different genotypes ofT.gondii and these findings have implications for the better understanding of the phenotypic differences among the examined T.gondii genotypes which in turn may contribute to the better control of toxoplasmosis.