Mechanism survey on Cell Senescence Induced by the knockdown of Cathepsin D through Quantitative Pro

• 作 者: Siyuan Su Ju Zhang Huiying Sun Xu Zhu Ningzhi Xu Siqi Liu Xiaomin Lou
• 机 构: Beijing Institute of Genomics,Chinese Academy of Sciences,Beijing,China
• 出 处: 第八届中国蛋白质组学大会
• 发表日期: 2013年11期
• 关键词: Quantitative proteomics SILAC LC-MS MS Cathepsin D Cellular senescence RNAi

Cathepsin D (Cath D) is one of the major proteases in the lysosome in eukaryotic cells.Various cell models have demonstratedthe enzymatic activity of cathepsin D is required to combat exogenous stresses.Based on our early observations,the knockdownof Cath D reduced proliferative and tumorigenic abilities of HeLa cells,induced significant G2/M arrest and cell senescence.