【摘 要】
：
目的：对外周血中食管鳞癌相关的7种miRNAs进行circRNAs及其靶基因的预测,分析其与circRNAs及靶基因间的相互作用.方法：根据前期进行系统综述和meta分析所获得的miRNAs,分别采用starBase及miRWALK对miR-18a、miR-21、mi-25、miR-31、miR-185、miR-223、miR-373的circRNAs和靶基因进行生物信息学预测,利用Cytosca
【机 构】
：
100069,北京市首都医科大学公共卫生学院流行病与卫生统计学系/北京市临床流行病学重点实验室
【出 处】
：
首都医科大学公共卫生学院第六届研究生学术论坛
论文部分内容阅读
目的：对外周血中食管鳞癌相关的7种miRNAs进行circRNAs及其靶基因的预测,分析其与circRNAs及靶基因间的相互作用.
方法：根据前期进行系统综述和meta分析所获得的miRNAs,分别采用starBase及miRWALK对miR-18a、miR-21、mi-25、miR-31、miR-185、miR-223、miR-373的circRNAs和靶基因进行生物信息学预测,利用Cytoscape软件对这7种miRNAs和预测所得到的circRNAs及相应的靶基因进行网络分析,并进一步通过DAVID软件对预测的靶基因进行通路分析.
结果：由starBase对这7种miRNAs预测的circRNAs数量分别为33、15、34、23、24、19和43个.其中SP140_hsa_circ_001735和SP140_hsa_circ_001283与miR-21、miR-25和miR-373等3种miRNAs相互作用,ISY1_hsa-circ_001859与miR-18a、miR-21和miR-31等3种miRNAs相互作用,ZNF264_hsa_circ_001634与miR-25、miR-31和miR-373等3种miRNAs相互作用.在miRWALK中7-8个以上数据库预测到的靶基因中,RSBN1基因是miR-25、miR-31、miR-185和miR-373等4种miRNAs相对应的靶基因,PDZD2基因是miR-18a、miR-21、miR-25和miR-31等4种miRNAs相对应的靶基因.RASA1基因是在2种预测范围内与miR-21、miR-31和miR-223等3种miRNAs相对应的靶基因.在7种miRNAs所预测的靶基因相关KEGG信号通路中,7-8个数据库以上共有的信号通路为TGF-beta信号通路、Cell cycle信号通路、ErbB信号通路、PI3K-Akt信号通路、Pathways in cancer和Signaling pathways regulating pluripotency of stemcells.
结论：本研究对外周血中食管鳞癌相关的miRNAs及其相互作用的circRNAs、靶基因和信号通路进行了生物信息学预测和网络分析,有助于进一步研究miRNAs在食管鳞癌发生、发展过程中的调控作用和方式.
其他文献
Objectives To figure the critical gap for pediatric reference intervals in China by validating three existing pediatric reference intervals(RIs)of complete blood count(CBC)respectively.Methods A total
Background Different confounder adjustment strategies were used to estimate odds ratios(ORs)in case-control study,.i.e.how many confounders that original studies adjusted and what the variables are.Th
Background Salt-sensitive hypertension(SSH)is a complex disease associated with many environmental and genetic factors.However,there are different medications to treat SSH that doctors are uncertain.O
Objectives To establish reference intervals of thyroid hormones for pregnant women based on hospital laboratory data using Hoffmann method and validate the feasibility of Hoffmann method.Methods This
Objectives To analyse trends in mortality and causes of death among under-5 children in Beijing,China between 1992 and 2015,and forecast under-5 mortality rates(U5MRs)for the period 2016-2020.Methods
Gestational hypercholesterolemia increases susceptibility to mitochondrial dysfunction.This study was aimed to determine whether the gestational hypercholesterolemia could induce mitochondrial dysfunc
LncRNA has been considered to play a crucial role in the progression of several diseases by affecting cell proliferation.However,its role in benzene toxicity remains unclear.Our study showed that the
Air pollution has been a major environment-related health threat.Most of the studies on PM2.5 toxicity have verified on the cardiovascular system and endothelial cells.However,researches on PM2.5-indu
Manganese(Mn)is an important environmental risk factor for Parkinsons disease (PD)and overexposure may result in manganism,a syndrome that closely resemble PD.Autophagy is an evolutionarily conserved
Although silica nanoparticles(SiNPs)have a promising application in biomedical fields,there is still a lack of comprehensive understand on genome-wide transcriptional analysis.This study is aimed to c