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One hundred fifty two actinomycetes were isolated form some radiation-polluted soil by using six different isolation media.Then sixty strains were chosen for 16S rDNA sequence and systematic analysis,which based on comparison results with their morphology and ARDRA.By 16S rDNA sequence analysis,the results showed that the strains were assigned to 12 recognized genera of actinomycctes,most of them fall within Streptomyces genus and a great deal of strains belonged to rare actinomycetes,that sugnested that there were a rich diversity of actinomycetes in radiation-polluted soil.