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Directed cell migration and lineage specific differentiation of neural stem cells are the keys to successful neurogenesis.Many guidance cues contribute to the regulation of neural stem cell migration and differentiation, we propose physiological electric stimulation is among such guidance cues in parallel with others including chemoattractant.We showed that physiological electric fields could trigger directed cell migration of neural stem cells, at not only 2D cell culture / ex vivo 3D culture, but also within spinal cord tissue post transplantation.Supermagnetic nanoparticle transfected neural stem cells showed normal cell viability and maintained multipotency, which can be tracked noninvasively using MRI post transplantation.Physiological electric fiends can also upregulate the neuronal differentiation from neural stem cells, in comparison with the non electric fields treated cells.Further more, such event is accompanied by organized alignment of axonal processes during the neuronal differentiation.Physiological electric stimulation therefore might potentially serve as a knovel approach in neural stem cells based replacement therapy in the treatment of central nervous system diseases.